Appendicitis can be diagnosed in time?
Appendicitis, one of the urgent surgery more often in children could be diagnosed early by detecting a protein in the urine, the researchers considered Proteomics Center for Pediatric Hospital in Boston as a biomarker for this disease.
Despite the evolution of diagnostic imaging techniques, recent studies show that between 3 and 30% of children suffer unnecessary appendicectomy, while 30-45% of those who do appendicitis are operated in the last minute.
While still primarily clinical diagnosis, physicians have sought more accurate laboratory methods to determine whether or not a patient has appendicitis.
Researchers led by Dr. Richard Bachur, head of the Emergency Hospital in Boston, decided to undertake a systematic approach and conducted a proteomics study to identify the most accurate biomarker for appendicitis.
In a first stage, examined 12 urine samples - six from patients with appendicitis, collected before and after appendectomy, and six from patients without appendicitis - 32 identifying substance that could be used as biomarkers. They were joined by other substances identified by other means, such as studies of gene expression, reaching a total of 57 possible candidates.
In a later stage, researchers have attempted to validate their usefulness in 67 children who were suspected appendicitis, of whom 25 came true diagnosis. The most accurate biomarker has proved leucine-rich alpha 2 glycoprotein (LRG). LRG increased levels can be detected by immunoblotting, which justifies the researchers hope to develop a rapid diagnostic test | 
